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水稻籼粳交F1花粉育性检测方法的研究──兼论其雌雄配子的育性问题

Study on Method of Measuring Pollen Fertility of F1 Between Indica and Japonic and Gamete Fertility

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【作者】 李自超鲁迪慧赵世绪王象坤

【Author】 LI Zichao; LU Dihui; ZHAO Shixu; WANG Xiangkun(College of Plant Science and Technology, China Agricultural University, Beijing 100094)

【机构】 中国农业大学植物科技学院!北京100094

【摘要】 以5个籼粳交组合的F1植株、1个低育的DH系和CPSLO17(对照品种)为材料,考察其结实率和花粉育性,花粉育性采用两种方法观察,I2-KI法和苏木精法,其中苏木精法是一种观察籼粳交花粉育性的新方法,用此法可直接在正常的成熟花粉中看到两个精子。结果表明,I2-KI法观察的花粉育性与结实率有显著差异,而苏木精法观察的花粉育性与结实率无差异。由此推论籼粳交F1不但存在雄配子部分不育而且还存在雌配子部分不育,两者的部分不育程度是相同的。

【Abstract】 Five F1s between indica and japonica rice, one DH line 96-5 with low panicle fertility and one variety CPSLO17 (Control) were used. Their panicle fertility was tested, and their pollen fertility was also done by two methods, I2-KI-stain and haematoxylin-stain. The latter was a new way by which the two normal sperms were observed in mature fertility pollen of F1 between indica and japonica rice. The results showed that the pollen fertility by I2-KI-stain and panicle fertility was significantly different. However, the pollen fertility by haematoxylin-stain and panicle fertility showed no difference. Therefore, the partly sterile appeared not only in male gamete but also in female gamete and they possibly were both equal.

  • 【文献出处】 中国水稻科学 ,CHINESE JOURNAL OF RICE SCIENCE , 编辑部邮箱 ,1997年03期
  • 【分类号】S511.103
  • 【被引频次】6
  • 【下载频次】165
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