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蜂斗叶的组织培养与快速繁殖
Rapid Propagation of Petasites japonicus by Tissue Culture
【摘要】 通过组织培养,蜂斗叶(PetasitesjaponicusMiq.)的叶柄和地下茎切段在MS+BA2mg/L的分化培养基上诱导出愈伤组织,并进一步分化出芽丛,切下2~3cm高的芽,移入1/2MS+NAA0.1mg/L的生根培养基中,生根率达100%。以再生苗的叶片或叶柄为外植体,在分化培养基上很快便可再形成愈伤组织并分化出芽。
【Abstract】 Butterbur is a kind of vegetable with high economic value. The petioles and tubers were cultured on MS+2mg/L BA media to produce callus and shoots. When the shoots grow to 2~3cm high,they were transplanted on 1/2 MS+0.1mg/L NAA rooting media. By using the leaf blade or petiole of regenerated buds as explant,callus and buds were produced more quickly.
- 【文献出处】 中国蔬菜 ,CHINA VEGETABLES , 编辑部邮箱 ,1997年06期
- 【分类号】S567
- 【被引频次】1
- 【下载频次】26