【摘要】 目的：本研究旨在探讨Ｔｅｔ对林丹—一种通过细胞内游离钙（［Ｃａ２＋］ｉ）介导大脑ｃｆｏｓ基因表达的神经毒剂—诱导的大鼠大脑ｃｆｏｓ基因表达的影响．方法：Ｎｏｒｔｈｅｒｎ印迹杂交，斑点杂交技术及双波长薄层色谱扫描分析．结果：大鼠口服林丹３０ｍｇ·ｋｇ－１１小时后大脑ｃｆｏｓ基因表达明显增强至１４６ｍｍ２，而在口服林丹前３０ｍｉｎ分别口服Ｔｅｔ１，２及４ｍｇ·ｋｇ－１，大鼠大脑ｃｆｏｓ基因表达被明显抑制（分别为８６，４０和３９ｍｍ２），其抑制程度呈剂量依赖关系．结论：Ｔｅｔ抑制Ｃａ２＋激动剂类神经毒剂—林丹诱导的大鼠大脑ｃｆｏｓ基因转录水平的表达．更多还原

【Abstract】 IM: To detect the effect of tetrandrine (Tet) on c fos gene expression in cerebrum induced by lindane, a neurotoxicant which activates Ca 2+ channels. METHODS: Northern and dot blotting, dual wavelength thin layer chromatography scanner, were used in this study. RESULTS: Lindane 30 mg·kg -1 given by intragastric gavage (ig) increased the expression of c fos gene to 146 mm 2 in rat cerebrum 1 h after treatment. Tet 1, 2, and 4 mg·kg -1 given by ig 30 min prior to lindane reduced c fos gene expression in a concentration dependent manner. Expressed genes reached only 86, 40, and 39 mm 2, respectively. CONCLUSION: Tet inhibited c fos gene expression in rat cerebrum induced by Ca 2+ agonist — lindane.更多还原