节点文献

苦豆子中生物碱含量测定方法新探

A New Method for the Determination of Total Alkaloid in Foxtail-like Sophora(Sophora alopecuroides)

  • 推荐 CAJ下载
  • PDF下载
  • 不支持迅雷等下载工具,请取消加速工具后下载。

【作者】 张建华乌云

【Author】 Zhang Jianhua and Wu Yun(Zhang Jianhua,Dept.of Biology Engineering,Inner Mongolia College of Farming and Animal Husbandry,Huhehaote)

【机构】 内蒙古农牧学院生物工程系!呼和浩特010018

【摘要】 苦豆子中生物碱具有一定水溶性,用氯仿难以提取完全。笔者采用水或pH=5.6的醋酸缓冲液作提取液,即将其中的生物碱转化成生物碱盐的方法,提取苦豆子中的生物碱。经DU-7紫外分光光度计测试,最大吸收波长为417nm,线性范围0~13μg/mL,变异系数0.65%,回收率:水提取为98.23%;醋酸缓冲液提取为98.72%,结果表明:测试数据准确,方法可行

【Abstract】 Alkaloids in Sophora alopecuroides L. were water soluble to a certain extent,but can not be completely extracted by chloroform. A method using water or acetic acid buffered water (pH = 5. 6)as extractant,after converting the alkaloids into their salts was studied. With the aid of a Du-7 UV-spectrophotometer,the maximum absorption wave length was found at 417 nm ;linear range within 0~13 μg/mL and variation coefficient 0. 65%. Recovery by water extraction was 98. 23 % and by acetic acid extraction was 98. 72%

【关键词】 苦豆子总生物碱提取法
  • 【文献出处】 中草药 ,CHINESE TRADITIONAL AND HERBAL DRUGS , 编辑部邮箱 ,1997年08期
  • 【分类号】R284
  • 【被引频次】38
  • 【下载频次】489
节点文献中: 

本文链接的文献网络图示:

本文的引文网络