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复方女贞子胶囊中齐墩果酸的含量测定

Determination of Oleanolic Acid Content of Compound N■zhenzi Capsule

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【作者】 毛丽珍徐世芳唐红芳张灵灵

【Author】 Mao Lizhen;Xu Shifang,et al(Mao Lizhen,Zhejiang Academy of Medical Sciences,Hangzhou)

【机构】 浙江省医学科学院!杭州310013浙江省宁波市第二医院

【摘要】 采用薄层扫描法测定复方女贞子胶囊中齐墩果酸的含量。以硅胶G为薄层吸附剂,环乙烷-丙酮-乙酸乙酯(4:2:1)为展开剂,10%硫酸乙醇为显色剂,用DS-930薄层扫描仪,在λS=530um,λR=680um波长处扫描。本法的平均回收率为99.22%,同一批样品平行试验6欢的相对标准偏差为3.14%。

【Abstract】 Content of oleanolic acid in compound Nuzhenzi capsule was determined by TLCS on plates of silica gel G and 0. 5% CMC-Na solution. The plates were developed in a solvent system of cyclohexane-acetone-ethyl acetate(4: 2: 1 )and colored with 10% sulfuric acid-ethanol solution- Oleanolic acid was detected in the double wavelength absorbance-reflectance mode at he(530nm)and referred at AR (680 nm). The assay is linear in the range of 1. 13~9. 04 μg for oleanolic acid. Mean recovery of the assay is 99. 22%.

  • 【文献出处】 中草药 ,CHINESE TRADITIONAL AND HERBAL DRUGS , 编辑部邮箱 ,1997年08期
  • 【分类号】R284
  • 【被引频次】10
  • 【下载频次】142
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