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HPLC法测定复方中成药桔红止咳冲剂中柚皮甙的含量

Quantitative Analysis of Naringin in Juhongzhike Chongji by HPLC

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【作者】 何燕萍; 王雅玲;

【Author】 He Yanping and Wang Yaling (Address:He Yanping,School of Pharmacy,Beijing Medical University,Beijing)

【机构】 北京医科大学药学院应用药物研究所!100083;

【摘要】 用HPLC对桔红止咳冲剂中的柚皮甙作了定量分析、样品用甲醇提取,在VarianKYWGC187μm(220min×4.6mm)柱上,用甲醇-1%醋酸(33:67)作流动相,各成分能很好分离,以标准曲线法测定了不同批号的10批样品中柚皮甙的含量、结果表明本法简便、快速、重视性好,测定回收率达100.1%,RSD为1.18%。

【Abstract】 Quantitative analysis of naringin in "Juhongzhike Chongji"was carried out by HPLC. Samples were extracted with methanol. An ODS column of Varian KYWG C18 7 μm (220×4. 6 mm ) and mobile phase methanol-1 % acetic acid (33: 67)were etnployed. Calibration curve method was used to determined the contents of naringin in ten samples.Results showed that the method is simple,quick and reproducible. The recovery was 100. 1% and RSD 1. 18%.

【关键词】 HPLC; 化桔红; 柚皮甙; 中成药;
  • 【文献出处】 中草药 ,CHINESE TRADITIONAL AND HERBAL DRUGS , 编辑部邮箱 ,1997年04期
  • 【分类号】R284
  • 【被引频次】8
  • 【下载频次】68
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