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HPLC法测定复方中成药桔红止咳冲剂中柚皮甙的含量
Quantitative Analysis of Naringin in Juhongzhike Chongji by HPLC
【摘要】 用HPLC对桔红止咳冲剂中的柚皮甙作了定量分析、样品用甲醇提取,在VarianKYWGC187μm(220min×4.6mm)柱上,用甲醇-1%醋酸(33:67)作流动相,各成分能很好分离,以标准曲线法测定了不同批号的10批样品中柚皮甙的含量、结果表明本法简便、快速、重视性好,测定回收率达100.1%,RSD为1.18%。
【Abstract】 Quantitative analysis of naringin in "Juhongzhike Chongji"was carried out by HPLC. Samples were extracted with methanol. An ODS column of Varian KYWG C18 7 μm (220×4. 6 mm ) and mobile phase methanol-1 % acetic acid (33: 67)were etnployed. Calibration curve method was used to determined the contents of naringin in ten samples.Results showed that the method is simple,quick and reproducible. The recovery was 100. 1% and RSD 1. 18%.
- 【文献出处】 中草药 ,CHINESE TRADITIONAL AND HERBAL DRUGS , 编辑部邮箱 ,1997年04期
- 【分类号】R284
- 【被引频次】8
- 【下载频次】68