【摘要】 用体外ＤＮＡ聚合酶Ｉ作用下ＤＮＡ合成的方法，研究ＨＨ０７Ａ的作用机制。结果表明，ＨＨ０７Ａ对ＤＮＡ聚合酶Ｉ催化下的ＤＮＡ合成有明显抑制作用，且在一定浓度范围内存在浓度依赖性。将药物与ＤＮＡ模板及ＤＮＡ聚合酶Ｉ分别预保温后，发现ＤＮＡ模板活性无明显改变，而酶的活性则受到显著抑制。提示ＨＨ０７Ａ对ＤＮＡ聚合酶Ｉ催化下ＤＮＡ合成的抑制是通过ＨＨ０７Ａ与ＤＮＡ聚合酶Ｉ直接作用而实现的。更多还原

【Abstract】 The effect of HH07A on DNA polymerase I in a cell free system was studied. The results showed that HH07A strongly inhibited the DNA synthesis catalyzed by DNA polymerase I in a dose dependent manner. Further results indicated that the DNA synthesis decreased significantly after DNA polymerase I was preincubated with HH07A. However, no change was found when DNA template was preincubated with HH07A. These results suggest that the inhibitory action of HH07A appeared to be on the enzyme molecules rather than on the DNA template.更多还原