【摘要】 近年来质谱离子化技术方面有两项重要成果:基质辅助激光解吸离子化(matrix-assisted laser desorptionionization,MALDI)和电喷雾离子化(electrospray ionization,ESI)。MALDI和ESI的应用使质谱在生物大分子研究方面取得重大突破。本文仅就MALDI的原理、特点、样品准备方法、基质的选择、仪器条件及其在生物大分子应用方面的最新进展进行简要的综述。更多还原

【Abstract】 In last few years, two important achievements of ionization techniques in mass spec-trometry have been developed: matrix - assisted laser desorption ionization (MALDI) and electrospray ionization (ESI). By these innovative techniques, mass spectrometry becomes one of the most important experimental tools for the analysis of biomacromoleculars. In this article, we briefly review the principles, characteristics, sample preparation, matrix selection, instrumentation of MALDI and its current progress in biomacromolecular analysis.更多还原