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节节麦×大麦杂种胚再生植株的细胞遗传学研究
Cytogenetics of Regenerated Plantsfrom Embryo Callus of the Hybrids betwen Aegilops tauschii and Hordeum vulgare
【摘要】 以节节麦(2n=14)为母本和大麦(2n=14)进行杂交,两组合平均结实率为3111%。对13个幼胚进行愈伤组织诱导培养,其中2个形成全能性愈伤组织进而分化出再生杂种植株。细胞学观察表明,杂种胚再生植株均是染色体加倍的节节麦-大麦双二倍体(2n=28)或染色体再加倍的多倍体。节节麦-大麦双二倍体在减数分裂中期Ⅰ染色体配对平均构型为1479Ⅰ+195Ⅱ+453Ⅱ'+001Ⅲ,表现为自交不育。杂种胚再生植株染色体数目再加倍的多倍体有少数产生了自交种子。
【Abstract】 Hybridizations betweenAegilops tauschii(2n=14) andHordeum vulgare (2n=14) were made using Ae. tauschii as female. The mean frequency of seed set was 3111% in two cross combinations, 13 hybrid embryos were cultured for inducing callus, of which 2 produced totipotent callus, and plants were regenerated from them. These plants were Ae. tauschii-H. vulgare amphidiploids with doubling chromosome number (2n=28) and octoploids with repeatedly doubling chromosome number (2n =50~56). The Ae. tauschii-H. vulgare amphidiploids were sterile and their average chromosome pairing at MI were 1479Ⅰ+195Ⅱ+453Ⅱ’+001Ⅲ.Some octoploid (2n =56) plants produced seeds after selfing.
【Key words】 Aegilops tauschii Cosson; Hordeum vulgare L.; Plant regeneration; Chromosome doubling; Gene sterile;
- 【文献出处】 遗传 ,HEREDITAS(BEIJING) , 编辑部邮箱 ,1997年01期
- 【分类号】Q343
- 【被引频次】9
- 【下载频次】39