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McAb-RIHA试验诊断血吸虫病的现场应用研究

A STUDY ON DIAGNOSIS OF SCHISTOSOMIASIS USING REVERSE INDIRECT HEAMAGLUTINATION (RIHA) WITH MONOCLONAL ANTIBODY

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【作者】 胡万富王之怀尹小梅王勇汪昊季虹唐为忠严自助

【Author】 Hu Wanfu;Wang Zhihuai;Yin Xiaomei;Wang Yong; Wang Hao;Ji Hong (Anhui Provincial Institute of Parasitic Diseases Wuhu 241001 )

【机构】 安徽省血吸虫病防治研究所!芜湖241001中国预防医学科学院寄生虫病研究所中国预

【摘要】 本文以单盲法在现场条件下对单克隆抗体反向间接血凝试验(McAb-RIHA)诊断血吸虫病的效果进行了观察,结果显示McAb-RIHA与Kato法粪检样本的检测总符合率达80.0%,与粪检阳性样本的符合率达83.9%;对首次粪检阳性而McAb-RIHA检测阴性的8份样本复查,发现3份为假阳性;对粪检阴性,McAb-RIHA检测阳性的样本,以Kato法复查(3张涂片),检出24.6%的阳性样本,是总样本粪检阳性率6.8%的3.6倍;与试验中平行设置的单克隆抗体斑点酶联免疫吸附试验(McAb-Dot-ELISA)的检测结果比较,两法的结果差异无显著意义。试验结果说明,McAb-RIHA在现场诊断血吸虫病具有较高的敏感性和准确性,该法操作简便、诊断快速,具有一定的实用价值。

【Abstract】 This paper reported the diagnostic effect for Schistosomiasis japonica using McAb-RIHA with the monoclonal antibody in single-blind in the field. The results showed that the coinci dence rate between McAb-RIHA and Kato-Katz method for all of identified samples was 80% and the coincidence rate of positive cases between McAb-RIHA and the stool examina tion was 83. 9%. 8 samples which were positive by Kato-Katz but negative by McAb-RIHA at first detection were checked again by Kato-Katz examination,the results showed that 3 of 8 samples were false positive. Of 57 samples which were negative by Kato-Katz but positive by McAb-RIHA at first detection, 14 were positive by Kato-Katz detection again. There was no significant differenoe of the results between McAb-RIHA and McAb-Dot-ELISA. It indi cated that McAb-RIHA for diagnosis of schistosomiasis in the field was sensitive,simple and fast,and more suitable fol use in the endemic areas.

【基金】 “八·五”攻关课题
  • 【文献出处】 中国血吸虫病防治杂志 ,CHINESE JOURNAL OF SCHISTOSOMIASIS CONTRAL , 编辑部邮箱 ,1997年03期
  • 【分类号】R532.2
  • 【被引频次】4
  • 【下载频次】16
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