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荧光素-番红花红T能量转移体系的研究及其作为核酸荧光探针的应用
Study on the Fluorescein Safranine T Energy Transfer Systems and Its Application as A Fluorescent Probe for Nucleic Acid
【摘要】 研究了以能量转移作为核酸荧光探针的可能性,提出利用能量转移体系测定DNA的新方法.该方法灵敏、快速,重现性好,DNA测定的线性范围是0.05~10mg/L,检测限为0.047mg/LCTDNA(n=11).
【Abstract】 The possibility of energy transfer between FL and ST systems as a fluorescent probe for deoxyribonucleic acid was investigated, and based on the energy transfer systems a novel fluorescence method for the determination of double strands DNA has been presented.This method is sensitive, rapid and reproducible. Linearity is observed in the range 0.05 ~ 10 mg/L CT DNA, the limit of detection is 0.047 mg/L CT DNA ( n =11).
【关键词】 荧光素-番红花红T;
能量转移;
DNA;
荧光探针;
【Key words】 Fluorescin Safranine T; Energy transfer; DNA; Fluorescent probe;
【Key words】 Fluorescin Safranine T; Energy transfer; DNA; Fluorescent probe;
【基金】 国家自然科学基金
- 【文献出处】 厦门大学学报(自然科学版) ,JOURNAL OF XIAMEN UNIVERSITY(NATURAL SCIENCE) , 编辑部邮箱 ,1997年06期
- 【分类号】O657.3
- 【被引频次】18
- 【下载频次】158