【摘要】 目的：了解ｐ５３及Ｃ－ｅｒｂＢ２、Ｃ－ｒａｓ、Ｃ－ｍｙｃ在卵巢粒层细胞瘤中的表达及与此瘤发生、发展的关系。方法：采用单克隆抗体免疫组化技术，对２５份卵巢粒层细胞瘤组织进行ｐ５３、Ｃ－ｅｒｂＢ２、Ｃ－ｒａｓ及Ｃ－ｍｙｃ表达的检测，分析基因表达与临床－病理指标及患者预后的关系。结果：２５例卵巢粒层细胞瘤中ｐ５３、Ｃ－ｅｒｂＢ２、Ｃ－ｒａｓ、Ｃ－ｍｙｃ的过度表达率分别为５６％、５６％、４８％、４８％。Ⅲ～Ⅳ期ｐ５３的过度表达高于Ⅰ～Ⅱ期；有残余瘤者较无残余瘤者显著增高。Ⅲ～Ⅳ期的Ｃ－ｅｒｂＢ２过度表达率显著高于Ⅰ～Ⅱ期者。高分化组的Ｃ－ｒａｓ基因过度表达率显著高于低分化组。未发现Ｃ－ｍｙｃ的表达与临床－病理各指标有关。两种及两种以上基因同时表达者显著高于单基因表达者。ｐ５３与Ｃ－ｅｒｂＢ２及Ｃ－ｍｙｃ过度表达间呈正相关。临床分期及Ｃ－ｅｒｂＢ２的过度表达是独立影响患者预后的因素。结论：ｐ５３及Ｃ－ｅｒｂＢ２、Ｃ－ｒａｓ、Ｃ－ｍｙｃ的表达对卵巢粒层细胞瘤的发生、发展及患者预后有一定的影响。更多还原

【Abstract】 Objectives:To determine the overexpressions of p 53 and C erbB 2,C ras,C myc in GCT of the ovary,and to analyse their correlations with the development of GCT. Methods:Overexpressions of p 53 and C erbB 2,C ras,C myc were determined by immunohistochemistry in paraffin embedded tumor tissues from 25 patients with GCT. Results:The incidences of overexpression of p 53 and C erbB 2,C ras,C myc were 56%(14/25),56%(14/25),48%(12/25),48%(12,25),respectively.p 53 overexpression rate in stages Ⅲ/Ⅳ was higher than that in stages Ⅰ/Ⅱ;patients with residual tumor after initial surgery was higher than those without residual tumor too.C erbB 2 immunoreactivity was higher in tumors with stages Ⅲ/Ⅳ than that in tumors with stages Ⅰ/Ⅱ.C ras overexpression was associated with tumor differentiation.The overexpression rates of more than two oncoproteins were higher than those with expression of one oncoprotein.p 53 overexpression correlated with overexpression of C erbB 2 and C myc.According to the FIGO staging and the C erbB 2 expression were independent variables for predicting patients’ prognosis. Conclusions:Overexpressions of p 53 and C erbB 2,C ras,C myc have some effects on carcinogenesis,tumor progression and prognosis of patients with GCT.更多还原