【摘要】 本实验采用滤纸和硅胶干燥法预处理水稻愈伤组织能明显提高愈伤组织的绿苗分化频率。其中以干燥处理4天、愈伤失水量在50％以上的培养效果较好。干燥处理能促进愈伤组织对矿物质元素的吸收、影响过氧化物同工酶的表达、降低脯氨酸的含量,而对细胞透性影响不大。更多还原

【Abstract】 Rice calli from mature embryos were dried with filter papers or silica gel. The results indicated that desiccation could significantly increase the regeneration frequency of the callus. The optical condition for plant regeneration was desiccation for 4 days with the filter paper treatment when more than 50% water in the callus was lost. Desiccation could also promote the absorption of mineral elements by the callus, affect the expression of the peroxidase isozyme and reduce the proline content. But it had no obvious effect on the ion penetrability of the cell membrane.更多还原