【摘要】 24-亚甲基胆固醇和谷甾醇用酵母基因突变型GL－7进行生物转化，在酵母22-位脱氢酶的作用下形成其相应的22-位去饱和的产物。用色谱、光谱以及与标准品对照等方法鉴定代谢产物的结构分别为菜子甾醇和豆甾醇。更多还原

【Abstract】 Incubations of 24-methylenecholesterol and sitosterol with yeast mutant GL-7 afforded their corresponding 22-desaturated products under the catalysis of sterol △22(33)-desaturase. The products were identified to be brassicasterol and stigmasterol respectively based on their chromatographic and spectral properties as well as by comparison with the authentic samples.更多还原