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HPLC示差折光分析法测定乳糖醇的含量

Determination of lactitol by HPLC with refractive index detector

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【作者】 陈悦; 陈青俊;

【Author】 Chen Yue and Chen Qingjun ((Zhejiang Institute for Drug Control, Hangzhou 310004)

【机构】 浙江省药品检验所!杭州; 310004; 浙江省食品质量监督检测站;

【摘要】 HPLC法测定乳糖醇的含量:采用Beckman碳水化合物柱,流动相为重蒸水,柱温90℃,示差折光检测器检测。最低检测限为10μg/ml(S/N=3),在0.25~4mg/ml的范围内呈良好的线性关系,r=0.9994.日内和日间相对标准差均小于3.0%。

【Abstract】 An HPLC method was used to measure lactitol. It was equipped with a refractive index detecor and a Beckman carbohydrate column. The column temperature was maintained at 90±0. 5 ℃ and the mobile phase was redistilled water. The results showed that the limit of detection was 10 μg/ml (S/N=3), the linear range was 0. 25~4 mg/ml (r = 0. 9994), and the precisions within a day or among d days all were less than 3. 0%.

【关键词】 HPLC; 示差折光检测; 乳糖醇;
【Key words】 HPLC; refractive index detection; lactitol;
  • 【文献出处】 西北药学杂志 ,NORTHWEST PHARMACEUTICAL JOURNAL , 编辑部邮箱 ,1997年04期
  • 【分类号】R927.2
  • 【被引频次】10
  • 【下载频次】171
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