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杜氏藻中多糖的化学研究(Ⅰ)
Chemical studies on the polysaccharide from Dunelinella salina(Ⅰ)
【摘要】 用0.2%稀碱溶液萃取杜氏藻提取出β-胡萝卜素后的残渣,经浓缩后用乙醇沉淀,得粗多糖,以Sevage法除去蛋白质,再经DEAT-纤维素及葡聚糖凝胶G-100柱层析,得杜氏藻多糖(简称PDS).多糖经薄层层析、纸层析、气相色谱及经甲基化后的色谱/质谱分析,获知有5种糖,即半乳糖、木糖、鼠李糖、葡萄糖、甘露糖,并测得各种糖的可能连接.
【Abstract】 PDS were isolated from Dunelinella salina residue after extraction of β carotene,and their components were identified by TLC,paper chromatography and GC/MS methods as the following:gaxactopyranose,xylopyranose,rhalnnopyranose,mannopyranose,glucopyranose.And their possible linkages were determined.
【关键词】 杜氏藻;
多糖;
柱层析;
甲基化;
【Key words】 Dunelinella salina polysaccharidecolumn chromatographymethylate;
【Key words】 Dunelinella salina polysaccharidecolumn chromatographymethylate;
- 【文献出处】 西北师范大学学报(自然科学版) ,JOURNAL OF NORTHWEST NORMAL UNIVERSITY(NATURAL SCIENCE EDITION) , 编辑部邮箱 ,1997年04期
- 【分类号】O636.1
- 【被引频次】3
- 【下载频次】105