【摘要】 从两株具有高亲和力及中和活性的抗人肿瘤坏死因子－α（ｈＴＮＦ－α）的单抗杂交瘤细胞株１Ｃ３、３Ｆ６中分别提取总ＲＮＡ，通过ＲＴ－ＰＣＲ扩增并克隆了抗体的ＶＬ、ＶＨ基因。核苷酸序列分析表明，所克隆基因均为抗体的ＶＬ、ＶＨ基因，并分别属于Ｋａｐｐａ链Ⅳ亚组和重链Ⅲ（Ｄ）亚组。此外，两抗体可变区分子模型及与ｈＴＮＦ－α的结合模型的建立及分析，为进一步的基因工程抗体研究及抗原－抗体相互作用机理的研究奠定了基础。更多还原

【Abstract】 Mouse anti hTNF α monoclonal antibody 1C3 and 3F6 displayed a high affinity and specificity for hTNF α The variable light and heavy chain region(V L and V H) genes from the two hybridomas were cloned and amplified with RT PCR The nucleotide sequence analysis indicated that the cloned genes encoded the variable light and heavy chain domains from mouse anti hTNF α respectively and belonged to Kappa chain Ⅳ subgroup and heavy chainⅢ(D) subgroup respectively Moreover,the construction and analysis of molecular model of two McAb variable region and binding model of hTNF α laid the foundation of the further researches of engineering Ab and mechanism of Ag Ab interaction更多还原