【摘要】 本文分别采用真核表达载体ｐＥＦ－ＢＯＳ及ｐＣＭＶ４构建含起始码和终止码的抗９．１Ｃ３分子单克隆抗体重链可变区基因重组表达质粒９．１Ｃ３ＶＨｐＥＦ－ＢＯＳ及９．１Ｃ３ＶＨｐＣＭＶ４，并将其分别免疫ＢＡＬＢ／ｃ小鼠。取免疫小鼠血清对Ｋ５６２细胞进行间接免疫染色和流式细胞仪分析表明，基因免疫小鼠体内可诱导９．１Ｃ３分子内影像类抗独特型抗体的产生。更多还原

【Abstract】 In this paper,the recombinant expression vectors 9 1C3 V H pEF BOS and 9 1C3 V H pCMV4 containing the complete open reading frame of 9 1C3 V H were constructed by using eukaryotic expression vectors pEF BOS and pCMV4 respectively BALB/c micc were immunized by using 9 1C3 V H pEF BOS or 9 1C3 V H pCMV4 The indirect fluorescent staining and flow cytometry analysis were performed, taking the serum from gene immunized mice as the first antibody and the K562 cells expressing 9 1C3 ligand molecules as the indicator cells The results show that anti idiotypic antibody bearing internal image type of 9 1C3 molecule can be detected in the serum from 9 1C3 V H pEF BOS or 9 1C3 V H pCMV4 vector immunized mice更多还原