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秋水仙素诱导的野生大豆根类细胞超微结构变化

THE ULTRASTRUCTURAL CHANGES INDUCED BY COLCHICINE IN ROOT MERISTEMATIC CELLS OF GLYCINE SOJA

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【作者】 杨茂成丛斌赵建华张丕方

【Author】 Yang Maocheng;Cong Bin;Zhao Jianhua;Zhang Pifang (Fudan University Shanghai 200433)

【机构】 复旦大学!上海200433上海市农业科学院!上海

【摘要】 萌发了4d的野生大豆种子经秋水仙素处理3d以后,根尖分生区细胞的超微结构发生了一些显著变化:许多质体环绕细胞核分布,这些质体中含有数量不等的淀粉粒;液泡的体积明显增大,有几个大液泡分布在细胞核周围;少数细胞中形成网状粗面内质网聚集体;细胞核所占细胞体积的比例减小,有的细胞核形状变得极不规则。从超微结构分析,这些细胞已具备了分化细胞的特征,而不再处于有丝分裂之中。

【Abstract】 Glycine soja roots were incubated in colchicine for 3 days after they had germinated for 4 days. Obvious ultrastructural changes in the cells of root meristem regionwere observed:cell nucleus was surrounded by plastids with several starch grains inside,the volume of vacuole increased,large and regularly-shaped vacuoles displayed arroundnucleus and throughout cytoplasm. Reticulate rough endoplasmic reticulum stacks couldbe detected occasionally. The shape of some nuclei became irregulsr,and the nucleus occupied a smaller portion of cell volume compared with those of dividing root cells without colchicine treatment. All these characteristics indicate that the cells treated withcolchicine undergo a differentiation pathway instead of being kept at meristematic state.

【关键词】 野生大豆根尖秋水仙素超微结构
【Key words】 Glycine sojaRoot tipColchicineUltrastructure
【基金】 国家自然科学基金;国家教委博士点基金
  • 【文献出处】 武汉植物学研究 ,JOURNAL OF WUHAN BOTANICAL RESEARCH , 编辑部邮箱 ,1997年01期
  • 【分类号】Q942
  • 【被引频次】18
  • 【下载频次】122
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