【摘要】 对国产２００１型血液荧光测定仪测定血液中锌原卟啉的方法进行了评价和规范化研究。结果表明：２００１型血液荧光测定仪测定血中锌原卟啉（ＺＰＰ）准确度和精密度较高，空气中相对湿度≤９０％，对测定无影响，方法的检测限为０．０２２５μｍｏｌ／Ｌ（每克Ｈｂ含０．１μｇ），标准曲线的线性范用０～３．３８μｍｏｌ／Ｌ（每克Ｈｂ含０～１５μｇ）。批内精密度ＲＳＤ＝０．２７％～１．９３％。准确度：血中ＺＰＰ标准物质的测得值及三个浓度都在给定值的不确定度范围之内。如血样不能立即测定时，可加肝素稀释液保存，４℃可保存３ｄ，－８℃可保存５ｄ。国产盖玻片其空白荧光值低于０．７９μｍｏｌ／Ｌ（每克Ｈｂ含３．５μｇ）者，可代替ＫＬＩ盖玻片。本方法准确度高、精密度好、快速、简便、建议推广应用。更多还原

【Abstract】 The authors studied the normalization and evaluation of the method detecting the zinc protoporphyrin (ZPP) by direct displaying blood fluorescent. The results revealed that this method was highly accurate and precise. The relative humidity not more than 90% had no influence to the results. The detection limit of this method was 0 1μg/g. The linear range of standard curve was 0～15μg/g, and the precision in batch RSD =0 27%～1 93%. On the accuracy side, the three concentration curving values of ZPP standard material in blood were all within the range of indefinition of given values. If the samples were not detected rapidly and the blood was diluted by heparin, it might be preserved at 4℃ for 3days, at -8℃ for 8days. The blank fluorescent value of glassy cover made in China is less than 3 5μg/g, Hb can substitute KLI glassy cover. This method can analyse one sample per minute, has advantage of rapidity, convenience, accuracy and reliablity.更多还原