【摘要】 <正> 多能硫杆菌（Thiobacillus versutus）是兼性化能自养细菌,在生理学和分类学上具有重要的地位,也是研究硫杆菌生理、生化、遗传学的理想材料。该菌通过卡尔文循环固定CO₂,其关键酶是1,5-二磷酸核酮糖羧化酶/加氧酶（简称RubisCO）。我们从多能硫杆菌中分离得到的RubisCO基因片段能够在大肠杆菌细胞中表达,说明自养细菌与异养细菌在基因表达方面是相似的。更多还原

【Abstract】 The chromosomal DNA of Thiobacillus versutus was hybridized with various heterologous ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO) gene as probes. Only Rhodobacter sphaeroides form I RubisCO gene showed homology with T. versutus. The RubisCO gene fragment of T. versutus was isolated using the RubisCO gene of R sphaeroides as a probe. And the RubisCO gene of T. versutus can express in E.coli cell.更多还原