【摘要】 香菇(Lentinula edodes)465菌株用添加啤酒发酵醪泥的培养基发酵培养10d后,菌丝体经洗涤、热水浸提、乙醇沉淀,再经DEAE-纤维素柱(CI—型)分离和Sephadex G—100柱层析纯化,得到白色絮状多糖LeBD1—1。经醋酸纤维薄膜电泳和HPLC检测纯度,LeBD1—1为均一成分。HPLC法测得分子量为130000Da,红外光谱测定其糖苷键为α型。经完全酸水解后用硅胶薄层层析和气相色谱法分析单糖组成,LeBD1—1中含D-甘露糖、D-葡萄糖、D-半乳糖、D-木糖和D-阿拉伯糖,各单糖摩尔比为2.45:1.00:0.03:0.08:0.10,是一种以含甘露糖为主的杂多糖。更多还原

【Abstract】 After the 465 strain of Lentinula edodes is fermented for ten days on the me dium with beer mess added to it, the mycelia are washed, extracted by hot water and precipitated by alcohol and then isolated by DEAE-cellulose column chromatography, and purification by Sephadex G-100 gel filtration. After LeBDl-1 is obtained, CAM electrophoresis and HPLC are used to determine its purity and they are proved that LeBD1 - 1 shows homogeneous. The HPLC method determines that its estimate molecular weights is 130000Da. The structure of LeBDl -1 is investigated by silica TLC, GC and IR spectroscopy. The results indicate that the LeBDl-1 was composed of α-linked D - Mannose, D -Glucose, D - Galactose,S -Xylose and D - Arabinose in the molar ratios of 2.45 :1.00: 0-03 : 0.08 : 0.10. It’s a heteropolysaccharide with mannose as its main sugar.更多还原