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戊型肝炎病毒抗体(抗-HEV)酶联免疫试剂盒的研制及其应用

Detection and Utilization of HEV Antibody ELISA

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【作者】 张萍张云涛杨宗模

【Author】 Zhang Ping ,Zhang Yuntao,Yang Zongmo (Lanzhou Institute of Biological Products,Lanzhou 730046)

【机构】 卫生部兰州生物制品研究所

【摘要】 应用合成HEV多肽作为包被抗原,按间接ELISA法,研制成HEV抗体检测试剂盒,并用于检测标本1054份,其中献血员样品200份,阳性率为1%;体检样品502份,阳性率为1.59%;医院病人样品352份,阳性率为10.8%。

【Abstract】 An indirect ELISA was developed for the detection of anti HEV.The polyvinyl microtiter plates were coated with HEV pepticies that comes from corporation YES in Canada.The secoad antibody is mouse anti human IgG.This ELISA kit was used to detect 1054 specimens.The results showed that the positive rate of anti HEV in donor is 1%,in ill men is 10.8%,in common people is 1.59%.

【关键词】 抗-HEV间接法阳性率
【Key words】 Anti HEV Indirect ELISA Positive rate
  • 【文献出处】 微生物学免疫学进展 ,PROGRESS IN MICROBIOLOGY AND IMMUNOLOGY , 编辑部邮箱 ,1997年02期
  • 【分类号】R512.6
  • 【下载频次】37
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