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超滤法和盐析法粗提基因工程干扰素其比活的比较

Comparison of Specific Activities of Recombinant Interferon Extraced by Ultrafiltration and Salt out

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【作者】 张春丽李才丛松葛桂霞周丽红付丽娟

【Author】 Zhang Chunli,Li Cai,Cong Song, et al (Changchun Institute of Biological Products,Changchun 130062)

【机构】 卫生部长春生物制品研究所

【摘要】 我们的工作是应用YF2PSK31R型超滤器超滤粗提基因工程干扰素,并与盐析法对比。超滤法简便快速条件温和,超滤后干扰素平均收率为93.95%±2.48%,盐析法则为75.93%±3.9%,前者比后者提高18.02%,差异显著。比活前为:4.455E6±7.58E5IU/mg蛋白,后者为:1.6117E6±2.079E5IU/mg蛋白,差异也是显著的

【Abstract】 Our work was extracting the recombinant interferon by ultrafiltration method and salt out method,then comparing their specific activity between them.The velocity of ultrafiltration is facter its effect is mildly and their operation is easy.The recovery ratios after ultrafiltration were 93.95%±2.48%,but after salt out they were 75.93%±3.9%,their different was significant.The specific activities after ultrafiltration were 4.455E6±7.58E5IU/mg protein,but after saft out they were 1.6117E6±2.075E5IU/mg protein,their different was also significant.

  • 【文献出处】 微生物学免疫学进展 ,PROGRESS IN MICROBIOLOGY AND IMMUNOLOGY , 编辑部邮箱 ,1997年01期
  • 【分类号】R392-33
  • 【被引频次】2
  • 【下载频次】70
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