【摘要】 目的：观察大鼠血清中细胞因子在急性胰腺炎发病机理中的作用。方法：检测血清中细胞因子和TNF-α，观察胰、肝、肺、肾的病理变化及胰腺细胞培养液中淀粉酶和PLA₂化。结果：在坏死性胰腺炎和水肿性胰腺炎大鼠血清中，IL-1β、IL-6、IL-2和TNF-α都明显高于正常对照组（P＜0.01），水肿性和坏死性胰腺炎的胰组织中4种细胞因子都高于正常胰腺组（P＜0.01），但两者之间未见明显差别。水肿性胰腺炎分离培养的胰腺细胞上清液中测出IL-6和TNF-α都高于正常胰腺组。结论:急性胰腺炎症过程中有多种细胞因子参与发病机理，其在体内放大作用造成胰腺本身在内的全身多器官功能损害，而且细胞因子的升高与疾病的严重程度相关。更多还原

【Abstract】 Background／Aims:To investigate of cytokines in the pathogenesis of experimental acute pancreatitis.Methods:SD rat acute pancreatitis models were examined:（1）serum IL-1β、IL-6、IL-12、TNF-α、mylase and PLA₂;（2）morphology and pathology of the pancreas、liver、lung、and kidney;（3）cytokines in isolated pancreatic acini cells.Results:The mean serum IL-1β、IL-6、IL-12 and TNF-α levers in the acute edematous pancreatitis（AEP） group and acute nacrotic pancreatitis（ANP）group are significantly higher than those in control group（P＜0.01）,especially more higher in the ANP group.Similar results of cytokines in the pancreatitic tissue are seen.The release of TNF-αand IL-6 is increased in the inflammatory pancreatic acini cells.Conclusions:The cytokines may play an important role in the pathogenesis of ANP,and their release are also closely related to the severity of acute pancreatitis.更多还原