【摘要】 利用伪狂犬病毒（Ｐｓｅｕｄｏｒａｂｉｅｓｖｉｒｕｓ，ＰＲＶ）湖北地方株胸腺核苷激酶（ＴＫ）基因和ｇＧ糖蛋白基因启动子（ＰｇＧ），构建了ＰＲＶ基因转移载体．通过β－半乳糖苷酶基因确定ＰｇＧ控制下外源基因的表达并筛选出表达β－半乳糖苷酶的ＰＲＶ重组病毒．采用ＰＣＲ对重组病毒进行了初步鉴定，证实外源基因可由构建的转移载体导入病毒ｔｋ基因中，重组的ＰＲＶ湖北地方株可用于表达外源基因．更多还原

【Abstract】 The expression of β lacZ controlled by PRV glycoprotein gG promoter(PgG), with partial tk sequences of PRV strain HS9304 on both sides, was confirmed. And PRV recombinant expressing β lacZ was produced by insertion of PgG β lacZ into tk gene of PRV strain HS9304. The results indicate that PRV strain HS9304 has the potential to be used as gene expressing vector. A transfer vector was constructed successfully with PgG and tk sequences which can be used to transfer foreign gene into viral genome.更多还原