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黑卵蜂寄主识别利它素的性质研究
ISOLATION AND CHARACTERIZZATION OF THE HOST RECOGNITION KAIROMONE OF TELENOMUS SPP.
【摘要】 通过盐析、柱层析和分子筛层析,从松毛虫雌蛾附腺中分离到一种SP-I组份,经生化方法检测鉴定,此组份是一种糖蛋白,分子量为1.35×105,等电点为4.2,蛋白质含量92%,糖含量8%.它能引诱松毛虫黑卵蜂和松茸毒蛾黑卵蜂在非天然寄主上的产卵检查、刺探行为,是一种黑卵蜂寄主识别利它素.
【Abstract】 A compound named SP Ⅰwas isolated from the extract of the female Dendrolimus punctants and partially putified by (NH 4) 2SO 4 Salting out,CM C cation exchange column chromatography,Sephadex G 200 gel filtration.It was obtained clearly in one band in SDS PAGE.The molecular weight of the compound was 1.35×10 5;it was a acidic glucoprotein(pI=4.2),and contained 92% protein,8% carbohydrate. The bioassay results revealed that it could induced Telenomus dendrolimusi and T.dasychiri Chen et Wu to examine and to probe the artiticial host egg coated with SP Ⅰ,the host recognition kairomone.
【Key words】 dendrolimus punctatus; accessory gland; Telenomus sp.; host recognition kairomone; glucoprotein;
- 【文献出处】 武汉大学学报(自然科学版) ,Journal of Wuhan University(Natural Science Edition) , 编辑部邮箱 ,1997年06期
- 【分类号】S476.3
- 【被引频次】7
- 【下载频次】78