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趋磁细菌研究 Ⅳ.好氧趋磁细菌HM-1的分离及生物学特性研究

ISOLATION OF A AEROBIC MAGNETOTACTIC BACTERIUM HM 1 AND STUDIES ON ITS BIOLOGICAL CHARACTERSTICS

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【作者】 章勇良卫扬保杨清香

【Author】 Zhang Yongliang,Wei Yangbao,Yang Qingxiang (College of Life Sciences,Wuhan University, Wuhina 430072,China)

【机构】 武汉大学生命科学学院

【摘要】 从黄石磁湖底部泥水样中分离到一株杆状好氧趋磁细菌HM-1,在pH7.0~8.0、30℃振荡培养的条件下培养时,每升培养基含4g柠檬酸,0.5g硝酸钠和50mmol·L-1FeSO4,分别是它生长和磁小体合成的最适碳源、氮源和铁源;此菌为G-,氧化酶和过氧化物酶阳性,不积累聚-β-羟丁酸,G+C的摩尔分数为64.08(HPLC);磁小体含一种矿质元素Fe.

【Abstract】 A rod shaped magnetic bacterium was isolated from freshwater sediment and a pure culture was obtained. The organism was cultured in a chemical defind medium containing citric acid (4 g·L -1 ), nitrate (0 5 g·L -1 ) and FeSO 4 (50 mmol·L -1 ) as the carbon, nitrogen and iron soures respectivelly. This magnetotactic bacterium was G - and able to form white colonies on ager plate and grow in O 2 contained atmosphere (shaked at 200 r·min -1 ). In this condition, it synthsised magnetosomes, showed catalase and oxidase activities. Microaerophili condition was influeuce for its growth and magnetosomes formation. Intracellalar poly β hydroxybutyrate is not formed. The X G+C of the DNA is 64.08 (HPLC). The entrgy profiles of X ray showed by electron excitation in TEM indicating that its magnetosome consists of one mineral element Fe only.

【基金】 国家自然科学基金
  • 【文献出处】 武汉大学学报(自然科学版) ,Journal of Wuhan University(Natural Science Edition) , 编辑部邮箱 ,1997年06期
  • 【分类号】Q939.1
  • 【被引频次】22
  • 【下载频次】238
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