【摘要】 利用云南红豆杉(Taxus yunnanensis)细胞悬浮培养的最佳培养条件,进行了细胞10L规模的发酵培养研究。对细胞发酵培养过程中的pH值变化、糖利用率以及细胞生长周期等实验参数进行了测定。已进行的3次发酵培养的实验结果表明:培养细胞的生长率已达0.4g/L.d(即12.0g/L),培养细胞中紫杉酵的含量为0.119％(其中培养液中的紫杉醇含量约占42％)。初步建立了优化的云南红豆杉细胞大量培养系统。更多还原

【Abstract】 10 L scale cell fermentation culture was studied under the optimum culture conditions of cell suspension culture from Taxus yunnanensis. The test parameters such as pH value, URS and cell growth charge on cell fermentation culture were measured. The results of three duplication tests showed that the cell growth rate had reached 0. 4g/L. d(12. 0g/L)and the taxol content had reached 0. 12% respectively. About 42% of the total taxol content was in the medium and 58% was in the cells. Preliminary cell mass culture system was established.更多还原