【摘要】 研究以聚乙烯醇（PVA）吸附包埋法固定大肠杆菌最适宜的条件为：PVA10％，硅藻土2％，菌量2％。固定化大肠杆菌具有较好的活性，在适当条件下可保存40d以上，与顶空气相色谱法联合，测定大肠杆菌代谢产物CO2的抑制程度，以IC50判断毒性大小，旨在为实现水体生物毒性快速监测以及生物监测的仪器化、自动化提供科学依据。用该方法检测五种离子毒性，毒性（IC50）大小顺序为Hg2+（0．016×10-4）＞Co2+（0．562×10-4）＞Pb2+（3.162×10-4）＞Cd2+（5．01×10-4）＞Cu2+；并探讨硝基废水和硝基氯苯对固定化大肠杆菌活性的影响。更多还原

【Abstract】 The optimum concentrations for PVA immobilized bacteria E. colt were: PVA 10 a,Silicate2%,and bacteria 2%. Immobilized bacteria E. colt had good activity. They could live for more than 40 daysunder suitable conditions. Aiming at providing scientific proofs for exploring a rapid monitoring of thewastewater toxicity and bio-monitoring with instruments and automation,they were combined with a headspace gas chromatographic technique to determine the extent to which the environmental toxicants inhibitedcarbon dioxide (CO2) as a product of the bacteria E. coli metabolization and IC50, This method has been usedto test the toxicities of 5 ions,the results are put in an order of decreasing toxicity in terms of IC,. Value asfollows: Hg2+ (1. 6 ×10-6g/m3),Co2+ (5. 62×10-5g/m3), Ph2+ (3. 16×10-4g/m3), Cd2+(5. 01×10-4g/m3),Cu2+. the influence on the activity of immobilized bacteria E. Colt by nitrochlorobenzenes and wastewatercontaining nitro-compounds,was also discussed.更多还原