【摘要】 采用ELISA方法检测了30例癫痫患者外周血单个核细胞（PBMC）经脂多糖（LPS）诱生后培养上清中的细胞因子水平。结果发现，癫痫患者PBMC分泌肿瘤坏死因子（TNF-α）和白细胞介素6（IL-6）的水平分别为（5．705±0．702）μg／L和（177．60±54．50）ng／L，较正常对照组［分别为（3．118±0．373）μg／L和（78．67±6．47）ng／L］明显升高（均为P＜0．01），并且TNF－α与IL-6的升高存在明显的正相关（r＝0．705，P＜0．05）。应用Dot－ELISA及图像分析技术证实，癫痫患者PBMC表面人白细胞Ⅱ类抗原（HLA－Ⅱ抗原）的表达密度也显著高于正常对照组（P＜0．01）。结果提示，IL－6、TNF－α的分泌紊乱及HLA－Ⅱ类抗原的表达异常可能与癫痫的发病有关。更多还原

【Abstract】 The concentration of cytokines was examined in 30 patients with epilepsy by enzyme- linked immunosorbent assay (ELISA). Significantly higher TNF-α and IL-6 concentrations in supernatants of LPSinduced PBMCs from epileptics were demonstrated as compared with normal controls[(5. 705±0. 702) μg/L vs (3. 118±0.373) μg/L,P<0.01 and (177. 60±54. 50) ng/L vs (78. 67±6.47) ng/L,, P<0.01, respectively]. Besides, a significant positive correlation was found between the elevated levels of IL-6 and TNF-α secreted by LPS-induced PBMCs in epileptics. Expression of HLA class Ⅱ antigen on PBMCs from epileptics was found to be significantly increased as shown by Dot-ELISA. These data suggest that the disorder of IL6 and TNF-α seeretion as well as abnormality in HLA class Ⅱ antigen expression on PBMCs may be involved in the pathogenesis of epilepsy.更多还原