【摘要】 为了探索慢性缺氧性肺动脉高压与G蛋白α亚基的关系，作者应用慢性低压缺氧方法（氧浓度10％，缺氧30～40d）建立肺动脉高压动物模型，再用核酸分子杂交方法测定大鼠肺组织Gα（G(αi-2)、G(αi-3)、G(αs)）mRNA水平。结果表明，慢性缺氧性肺动脉高压大鼠肺组织GαmRNA水平与对照组比较，G(αi-2)、G(αi-3)分别为对照组的1．93和2．05倍;而Gαs无变化。提示，大鼠肺组织G(αi-2)、mRNA与G(αi-3)mRNA的增加，可能在慢性缺氧性肺动脉高压发病中起重要作用。更多还原

【Abstract】 To evaluate the relation between G proteins and hypoxic pulmonary hypertension (HPH), a stable rat model of HPH was established with chronic hypobaric hypoxia (10 % O2, 30-40d). By the use of dot-hybridization, the mRNA levels of α-subunits of G protein (Gαi-2, Gαi-3, Gαs) were examined in the lungs of rats before and after chronic hypoxia. The levels of Gαi-2,, Gαi-3, mRNA in HPH rats were 1. 93- and 2.05-fold respectively as high as those in normal rats, while there was no significant change in Gαs mRNA- The increased expression of Gαi gene might play an important role in hypoxic pulmonary hypertension.更多还原