【摘要】 茶卷蛾质型多角体病毒核酸（ＨｍＣＰＶＲＮＡ）用热酚法从提纯的病毒多角体中提取。紫外吸收光谱测定最高最低的吸收值分别在２６０ｎｍ和２３０ｎｍ处。经测定其Ｔｍ值为７９℃，在３％聚丙烯酰胺凝胶中，经电泳ＨｍＣＰＶＲＮＡ可分离得到１０条基因片段，各片段大小为０．３４×１０６～２．５５×１０６，总分子量为１５．１５×１０６，其ＰＡＧＥ图型与ＣＰＶ属代表种ＢｍＣＰＶＲＮＡ有较大差异，试验表明ＨｍＣＰＶ与ＢｍＣＰＶ属不同ＰＡＧＥ型。更多还原

【Abstract】 The Cytoplasmic Polyhedrosis Virus of Homona maganima is an insect reovirus. Its genome was extracted from the purified polyhedra of CPV with hot-phenol method and the unwinding temperature value(T m) is 79℃, which indicated that the genome was double stranded RNA. 10 bands were resolved when the viral genome was run in 3% polyacrylamide gels. The mol·wt·of the RNA fragment ranged from 0.34×10 6 to 2.55×10 6 D, and total was 15.15×10 6 D.更多还原