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CMP对HPBL的促诱生IL-6效应及IL-6中间体制备

Interleukin-6 Stimulation Effect of CMP on HPBL and Preparation of Intermediate Forms of IL-6

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【作者】 金苏华杨吉成盛伟华张云汤建平

【Author】 Jing Siyhua ;et al (Gene Engineering Unit of Suzhou Medical College,215007)

【机构】 苏州医学院基因工程研究室!215007

【摘要】 用羧甲基茯苓多糖(CMP)预处理人外周血淋巴细胞(HPBL)后经PHA或(和)ConA刺激的促诱生组IL-6效价,结果比无CMP的PHA或(和)ConA刺激的常规诱生组高0.5~0.8倍(p<0.01),尤以CMP+PHA+ConA促诱生IL-6效果最佳(p<0.01);而且可达生物制剂的药用标准,经临床试验证明安全有效。

【Abstract】 Huam peripheral blood lymphocytes (HPBL) were pretreated with carboxymethyl-pachymaran(CMP) and than inducers of PHA and/or ConA were added. The IL- 6 titers in the stimulatinghters induction groupe in which CMP was added in coha with PHA and/or ConA were 0. 5~0. 8times higher than that in the groups in which the conventional inducers of PHA and/ar ConA wereused without CMP(P<0. 01) , the highest of which was that in the stimulating induction group inwhich CMP+PHA+ConA were added (P<0. 01). The intermediate forms of IL- 6 produced withmethod of stimulating induction CMP possess high titers of IL- 6 and meet the pharmautical criteriaof bilogical products after tested through various peters and have been Proved indeed safe andefficient clinically.

  • 【文献出处】 苏州医学院学报 ,ACTA ACADEMIAE MEDICINAE SUZHOU , 编辑部邮箱 ,1997年04期
  • 【分类号】R73-3
  • 【被引频次】1
  • 【下载频次】18
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