l-Aminocyclopropane-l-carboxylic acid (ACC) synthase is the key emzyme in the biosynthasis of ethylene. Totol RNA was isolated from ripe Hetau melon (Cucumis melo L.cv Hetau) fruits. Using oligo(dT)(15) as primer the first strand of cDNA was synthesized and a 0. 63 kb DNA fragment was obtained with PCR technique. This DNA frangment wascloned into Sma I site Of pUC19, then introducted into E. coli JM109. Sequence analysis of the recombinant PHMAS1 demonstrated that a fragment of DNA encoding ACC synthase was...
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