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小鼠Sry基因和Sox基因的克隆分离与鉴定

Isolation and Analysis of the Mouse Sry and Sox Genes

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【作者】 张晓群王申五陈东陈永福陆道培

【Author】 Zhang Xiao Qun Wang Shen Wu Chen Dong  Chen Yong Fu  Lu Dao Pei(Institute of Hematology, People′s Hospital,Beijing Medical University,Beijing 100044) (National Agrobiotechnology Laboratory, Beijing Agricultural University)

【机构】 北京医科大学人民医院血液研究所

【摘要】 为了深入探讨性分化分子机制,构建了小鼠基因文库以SRY探针筛选,分离到了两组阳性克隆,一组含EcoRⅠ/3.5kb,此片段中载有小鼠Y染色体上的Sry基因.另一组含EcoRⅠ/3.0kb或SalⅠ/3.0kb,此片段有小鼠Y染色体之外的Sox基因

【Abstract】 An EcoRⅠor SalⅠ3.0 kb fragment and EcoRⅠ3.5 kb fragment from the positive clones screened by SRY probe in male mouse genomic library and forced cloning library were described respectively.The 3.0 kb fragment containing SRY box is located outside of Y chromosome mamely the Sox gene and the Sry gene of Kun Ming white mice is included in the 3.5kb fragment.

  • 【分类号】Q781
  • 【被引频次】5
  • 【下载频次】138
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