【摘要】 应用快速酶联免疫法（ELISA）及Northern印迹杂交法研究了博莱霉素（BLM）同系物诱导癌基因表达的作用．通过检测P21和c－myc蛋白表达的改变和药物在RNA的转录水平上对癌基因表达的影响，证明BLM能够抑制c－myc基因的表达．这种抑制作用不仅发生在蛋白质的翻译水平，而且可能发生在RNA的转录水平上．BLMA6及A2对Ras基因亦有极显著的抑制，提示其亦为以p21蛋白为靶点的抗癌抗生素．A6、A2与A5之间的区别提示在同系物之间可能存在不同的抗癌机理更多还原

【Abstract】 The study on gene expression changes induced by Bleomycin(BLM) analogues hasbeen reported. BLM analogues can effectively supress the c-myc oncogene expression on bothtranslation and transcription levels based on ELISA system and Northern blotting. Ras oncogenesuppressions induced by BLM A2 and A6 are remarkable, which indicates that they are p21-targeting anticancer antibiotics. The difference between BLM A2 and A6 and BLM A5 suggest thattheir anticancer activities may result from different mechanisms.更多还原