【摘要】 介绍了反相高效液相色谱法测定人的胃液中甲硝唑含量的方法。服药后插管抽取胃液，试样用无水乙醇沉淀蛋白，离心，过滤，进样测定。方法的线性范围为２．５～２００ｍｇ／Ｌ，最小检知量为０．４ｍｇ／Ｌ，平均回收率为９６．０％，变异系数为３．０７％。更多还原

【Abstract】 Metronidazole (MTZ) is the first chosen drug of anti anaerobe which was recommended by World Health Organization. In this paper a high performance liquid chromatographic (HPLC) method has been developed to determine the concertration of MTZ in human gastric juice. Gastric juice was obtained by intubation after taking drug, then the sample was precipitated with ethanol to remove proteins, centrifuged, filtered and finally assayed by HPLC. The operating conditions were reversed phase C 18 column(4mm×150mm), CH 3OH∶KH 2PO 4(5mmol/L, pH 4.0)=10∶90 mobile phase with flow rate of 1.5mL/min and detection wavelength at 320nm. The standard working curve was Y=-1.63+1.15X, with r=0.9999, n=7. The linear range was 2.5 200mg/L. The detection limit was 0.4mg/L. The average recovery was 96.0% and the coefficient of variation was 3.07%. This method can be applied to guide clinical therapy and pharmaceutical research.更多还原