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迷走神经结状神经节与心肌细胞联合培养的活细胞观察
OBSERVATION OF THE LIVING CELLS IN CO-CULTURE OF NODOSE GANGLIA AND CARDIAC MYOCYTES
【摘要】 用新生大鼠的连走神经结状神经节与心肌细胞进行联合培养。在相差显微镜下观察了神经元的生长以及神经一肌肉连接的形成.结状神经书与心肌细胞联合培养72~96h可观察到神经突起的终未终止于搏动的心肌细胞表面.神经节组织块周围有许多突起在心肌细胞表面相互交织成网状.交叉的突起相互粘连在一起,终止于抢动的心肌细胞表面的一个突起移动可牵动邻近的交叉突起同.本研究首次建立了在体外培养中具有结状神经节神经支配的心肌细胞模型.
【Abstract】 Nodose ganglia were added to newborn rat cartdiac muscle cell cultures. Neuronal growth and the formation of neuromuscular junctions were observed with phase contrast microscope. In nodose ganglion-myocyte co-cultures, the axons terminated on the contracting cardiac muscle cells between 72 and 96 hours of culture age. Axons would cross each other,hence displacement of a terminal axon on a contracting cardiac muscle cell would also oscillate the proximally crosing axonal network. These studies are the first to establish the nodose ganglial innervation of isolated card iac muscle cells in vitro.’ (Figures 1~ 3 on plate l2)
【Key words】 nodose ganglia; cardiac myocytes; co-culture; neonatal rat;
- 【文献出处】 神经解剖学杂志 ,CHINESE JOURNAL OF NEUROANATOMY , 编辑部邮箱 ,1997年01期
- 【分类号】R329
- 【被引频次】3
- 【下载频次】74