【摘要】 为了克隆和研究旋毛虫保护性抗原及特异性诊断抗原基因，我们用异硫氰酸胍从旋毛虫肌肉或幼虫中分离总RNA，用PolyATtractmRNA分离系统统化mRNA，以NotIprimeradapter为引物合成双链cDNA，加SalIadapter,与λgt22A在体外进行连接包装并转染大肠杆菌Y1090r－，构建cDNA文库。结果获得5×105个重组噬菌体，重组率在90％以上．更多还原

【Abstract】 The paPer describes the preparation of mRNA and construction of cDNA library from Tricinella spiralis for the purpose to clone and analyse individivual gene and its products which could be as candidate for development of diagnositic agents and vaccine for the disease. Total RNA was extracted by the method of using guanidinium thiocyanate phenol-chloroform and mRNA was separated by using Poly AT tract rnRNA system.cDNA was synthesized from poly(A)-rich mRNA and ligated and packaged in vitro into phage vector λgt 22A with Not I-Sal I arm by the primer and adapter methed. Alter the phages were traofected into E. coliY1090r-,it was propagated and achieved 5 ×105 recombinant phages with recombination rate over 90%.更多还原