【摘要】 重组人白细胞介素２（ｒｈＩＬ２）基因表达产物在大肠杆菌中以不溶性包涵体形式存在，经过菌体发酵和收集、超声破菌、包涵体抽提、稀释透析初步复性、反相高压液相色谱纯化，终产物纯度大于９９％。反相高压液相色谱不仅可以纯化ｒｈＩＬ２，且使产物的总活性提高７９～９倍，比活性提高１４～１８倍，达到１５×１０７ｕ／ｍｇ蛋白质，蛋白得率为５０％～５６％。结果表明，反相高压液相色谱具有纯化和显著提高ｒｈＩＬ２折叠效率的双重功效，为非ＳＤＳ变复性法生产ｒｈＩＬ２提供了简便有效的方法更多还原

【Abstract】 Recombinant human interleukin 2(rhIL 2) was expressed as inclusion bodies(IB) in E.coli . By means of fermentation, bacteria collection and breakage through sonication, IB solubilization, initial dilution dialysis renaturation, purification by reverse phase high performance liquid chromatography (RP HPLC), the purity of purified rhIL 2 was more than 99%. RP HPLC could not only purify rhIL 2 but also raise its total activity and specific activity to 7.9～9.0 and 14～18 times respectively. The specific activity of purified product reached to 1.5×10 7u/mg, and the protein recovery was 50%～56%. The results proved that RP HPLC has twofold function for purification and notably raising refolding efficiency of rhIL 2,which provided simple and useful parameters for production of rhIL 2 by non SDS denaturation and renaturation method.更多还原