【摘要】 菠菜放氧的ＰＳＩ核心复合物经０．８ｍｏｌ／ＬＴｒｉｓ－ＨＣｌ（ｐＨ８．０）洗涤后，用温和的非离子去垢剂ＤＭ和高浓度的ＬｉＣｌＯ４增溶，再经ＤＥＡＥ－Ｔｏｙｏｐｅａｒｌ－６５０Ｓ离子交换柱层析分离，可得到ＰＳＩＩ核心天线４３ｋＤ叶绿素ａ结合蛋白（ＣＰ４３）。ＳＤＳ－ＰＡＧＥ显示一条４３ｋＤ蛋白质带。根据Ａｒｎｏｎ法和Ｍａｒｋｗｅｌ法的结果表明，每个蛋白质分子结合有２０～２１个分子的叶绿素ａ。室温条件下，ＣＰ４３在红光区具有６７１ｎｍ的最大吸收峰和６８３ｎｍ的荧光发射峰，以及Ｗ型的圆二色信号，表明其处于较为天然的状态。文中还制备并鉴定了对ＣＰ４３特异的抗血清。更多还原

【Abstract】 The core antenna CP43 of photosystem II was purified from the PSII core complex of spinach by DEAE Toyopearl 650S anion exchange chromatography, using the mild nonionic detergent β dodecyl maltoside and high concentration of LiClO 4. At room temperature, the purified CP43 has a maximum absorption at 671 nm, a fluorescence maximum at 683 nm and doublet CD signals which indicated the presence of excitonic interactions between some chlorophylls. The pigment content of the CP43 was 20-21 Chl a per CP43 polypeptide as determined by a spectroscopic method. The results suggested that the purified CP43 was still in a native state. Moreover, the specific antiserum was obtained and identified.更多还原