【摘要】 错配碱基套式PCR RFLP检测K ras癌基因第 1 2位密码子点突变 ,并与一步法PCR RFLP作比较 .结果显示套式PCR RFLP可检测出 50 0细胞中的一个突变细胞 ,比一步法PCR RFLP分析的敏感性提高了 1 0 0倍 .利用该方法检测纤维支气管镜收集的标本中的突变细胞 ,结果发现 9例肺腺癌中有 5例发生了K ras癌基因第 1 2位密码子点突变 .提示该方法可行 ,值得推广应用 .更多还原

【Abstract】 An approach to detect K-ras oncogene mutated at codon 12 was devised by using a nested polymerase chain reaction strategy based on mismatched primers. A titration experiment showed that the sensitivity obtained by use of the nested PCR was 1/512. This represented the ability to detect one cell heterozygous for K-ras codon 12 mutation over 500 normal cells and was 100-fold greater than that obtained after one-step PCR. Application of this assay to 9 patients with adenocarcinoma of the lung showed 5 cases containing one or more K-ras mutation at codon 12. It is suggested that the method was practicable and worthy of application and dissemination.更多还原