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快速测定有机磷中毒生物传感器研究

STUDY ON BIOSENSOR OF ORGANOPHOSPHORUS TOXICATE

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【作者】 冯德荣孙士青周万里朱思荣李智红

【Author】 Feng Derong et al (Bioeogy Institute ,Shandong Acadany of Sciences,Jinan))

【机构】 山东省科学院生物研究所

【摘要】 采用固定化胆碱氧化与过氧化氢电极构成酶电极生物传感器测定血清或全血中胆碱酯酶活性,反应时间40s,样品量50μL,经用化学法对比测定有机磷中毒动物全血的胆碱脂酶活性,两法之间无显著差异。

【Abstract】 Choline Oxidase was immobilized at a hydrogen peroxide electrode and the enzyme electrode was used for the determination of acetylcholinesterase activity. Thelinear rang is 0 ̄200mg/L with a response time of 40 seconds and 50μl sample amount. There was no significante difference between biosensor and chemical method on analysis of acetylcholinesterase activity.

  • 【分类号】Q55
  • 【被引频次】1
  • 【下载频次】60
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