【摘要】 葡萄组织培养技术，特别是胚、胚珠培养技术在无核葡萄育种研究中发展迅速。利用无核葡萄品种作母本，特别是利用假单性结实的无核葡萄品种作母本，用无核品种作父本进行杂交，合子胚还未败育时，采用胚珠培养技术可以得到杂种实生苗。这种杂种实生苗无核比例较高，可以达到８２％，理论上可达１００％。选择合适的亲本，掌握无核葡萄胚珠败育时期，培养基激素的选择，以及如何促进胚的萌发，是正确利用这项技术的关键。更多还原

【Abstract】 Great progress has been made in the application of in vitro culture,especially embryo culture and ovule culture,in seedless grape breeding When hybridization using seedless grape cultivars as parents,in particular stenospermic seedless genotypes as female pareat,hybrid seedlings could be obtained by means of ovule culture before the hybrid embryos lose their fetility.The proportion of seedless could be as high as 82% as reported and even reach 100% in theory.The key issues of ovule culture include the selection of suitable parents,the knowledge of ovule abortion period,the selection of hormones in culture medium and the promotion of embryo germination.更多还原