【摘要】 将长２～３ｃｍ的小麦幼穗剪成２～３ｍｍ长的小切段接种于添加２，４－Ｄ、ＮＡＡ的ＭＳ培养基上，当愈伤组织直径达５ｍｍ左右时，不加分割直接转移到分化培养基上培养，获得５４个试管变态小穗，其颖片和小花柄明显伸长，呈簇生状或分枝状，比正常的大２～８倍，花器发育完全。在无菌条件下授以黑麦花粉，产生１１粒杂交种子。经离体胚培养，获得８株试管幼苗。更多还原

【Abstract】 Young wheat spikes of 2～3 cm in length were cut into 2～3 mm segments,which were then inoculated on the MS induction medium plus 2 mg·L -1 2,4 D,0.5 mg·L -1 NAA, 30 g·L -1 sucrose.When the induced Calli grew into about 5 mm in diameter,they were transferred directly onto differentiation mediam,from which 54 abnormal spikelets were obtained.These spikelets were fasciculate or branchlike,had acomplete floral organs which are 2～8 times larger than normal spikelets insize,and their glumes and rachillaes also became obviosly longer. Pollinated with rye pollens under aseptic condition the test tube spikelets yielded 11 hybrid seeds,from which 8 plantets were obtained through embryo culture.更多还原