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两种蛋白质对精子冷冻保护的影响
Comparison of the abilities of two proteins for cryopreservation of human spermatozoa
【摘要】 本文对蛋黄及白蛋白两种蛋白质在精子冷冻保护中的作用进行了比较,结果显示:尽管两种蛋白质对精子冷冻复苏后的活动率的影响没有明显的差异性,但在A23187诱导,FITC-PSA染色的精子顶体反应测试中,蛋黄显示出相对较好的保护作用;在临床人工授精效果的观察中,使用蛋黄保护剂的精子呈现了较高的怀孕率。
【Abstract】 Two proteins, egg yold and bovine serum albumin, in cryoprotective medium were assessed for efficacy of cryopreservation of human spermatozoa. The results indicated that both were equivalent for post-thaw motility of sperm. However, with FITC-PSA staining, a significantly higher number of acrosome-reacted spermatozoa were noted from yolk medium. The pregnancy rate of artificial insemination also supported the suggestion of acrosome reaction evaluation.
【关键词】 人精子;
冷冻保护;
顶体反应;
人工授精;
【Key words】 Human sperm; Cryopreservation; Acrosome reaction; Artificial insemination;
【Key words】 Human sperm; Cryopreservation; Acrosome reaction; Artificial insemination;
- 【文献出处】 男性学杂志 , 编辑部邮箱 ,1997年04期
- 【分类号】R321.1
- 【被引频次】1
- 【下载频次】61