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两种蛋白质对精子冷冻保护的影响

Comparison of the abilities of two proteins for cryopreservation of human spermatozoa

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【作者】 王学本麦庭福李科茂张伟铭吴伟雄

【Author】 WANG Xueben, MAI Tingfu, LI Kemao, ZHANG Weiming, Wu WeixiongInstitute of Guangzhou Family Planning (Guangzhou 510630)

【机构】 广州市计划生育科研所

【摘要】 本文对蛋黄及白蛋白两种蛋白质在精子冷冻保护中的作用进行了比较,结果显示:尽管两种蛋白质对精子冷冻复苏后的活动率的影响没有明显的差异性,但在A23187诱导,FITC-PSA染色的精子顶体反应测试中,蛋黄显示出相对较好的保护作用;在临床人工授精效果的观察中,使用蛋黄保护剂的精子呈现了较高的怀孕率。

【Abstract】 Two proteins, egg yold and bovine serum albumin, in cryoprotective medium were assessed for efficacy of cryopreservation of human spermatozoa. The results indicated that both were equivalent for post-thaw motility of sperm. However, with FITC-PSA staining, a significantly higher number of acrosome-reacted spermatozoa were noted from yolk medium. The pregnancy rate of artificial insemination also supported the suggestion of acrosome reaction evaluation.

  • 【分类号】R321.1
  • 【被引频次】1
  • 【下载频次】61
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