【摘要】 利用野油菜黄单胞菌（Xanthomonascompestris）8004正、负调控胞外酶及多糖合成的基因片断分别经三亲本接合方法导入黄单胞菌NK01抗性突变株中，测定接合于四种胞外酶活力和多糖合成能力，确定该正、负调控基因对NK01的作用．结果表明，负调控基因作用明显，使NK01胞外多糖及胞外酶产量显著降低，正调控基因未见明显的胞外酶及多糖合成的增强作用．本文并对正、负调控基因的作用方式作了初步探讨．更多还原

【Abstract】 The gene fragments from Xanthomonas campestris 8004 involved in positive,negative regulation of synthesis of extracellular enzymes and EPS are transferred into Xanthomonas campestris NK01 by triparental conjugation respectively.The production of extracellular enzymes and EPS is assayed,and the effects of these genes on NK01 are detected.It shows that,the effects of negative regulation genes are obivous,and the extracellular polymers synthesis is signifficantly reduced.While the effects of positive regulation genes are not detectable.The interaction pattern between these genes is discussed preliminarily.更多还原