【摘要】 为分析人脂蛋白脂酶基因多态性，采用两对引物进行聚合酶链反应（ｐｏｌｙｍｅｎａｓｅｃｈａｉｎｒｅ－ａｃｔｉｏｎ，ＰＣＲ）扩增人脂蛋白脂酶（ｌｉｐｏｐｒｏｔｅｉｎｌｉｐａｓｅ，ＬｐＬ）基因内含子６区和８区的ＰＶＵⅡ（１８９ｂｐ）和ＨｉｎｄⅢ（７１５ｂｐ）位点特异性片段。分别用限制性内切酶ＰＶＵⅡ和ＨｉｎｄⅢ酶解上述的ＰＣＲ产物。琼脂糖凝胶电泳后分离上述的酶解片段进行多态性分析。结果１０１名正常健康人ＬｐＬ的等位基因频率分别是Ｐ１６７．８％，Ｐ２３２．２％；Ｈ１７７．２％，Ｈ２２２．３％。此法简便、灵敏，且结果可靠。更多还原

【Abstract】 Using technique of polymerase chain reactionrestriction fragment lengths polymorphism (PCRRFLP), we detected the genotypes of lipoprotein lipase in 101 normal subjects. First amplified the specific base pairs of 189bp and 715bp in intron 6 and 8 region of human lipoprotein lipase (LpL) gene. Then we analysed genotypes using agarose electrophonesis after they were digested by PVUⅡ and HIND Ⅲ endonuclease. It shows that the technique of PCRRFLP was a useful measure to detect the genotypes of LpL. LpL gene was polymorphism, the frequence of LpL gene were H 1 777% H 2 22.3%, P 1 67.8%, P 2 32.2% respectively.更多还原