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水稻不同抗白叶枯病基因抗谱鉴定及在杂种F1代的表达
REACTION PATTERN OF VARIOUS RESISTANT GENES IN RICE TO CHINESE PATHOTYPES OF XANTHOMONAS ORYZAE PV. ORYZAE AND THEIR EXPRESSION IN HYBRIDS
【摘要】 测定了12个水稻抗白叶枯病基因对中国白叶枯病菌7个致病型的10个菌株的抗性反应。结果表明:Xa-4、xa-5、Xa-7、xa-13和Xa-21等5个抗病基因在苗期、分蘖期和孕穗期对病菌侵染均表现为广谱抗病反应。进一步将3个带显性广谱抗病基因的材料IRBB4(Xa-4)、IRBB7(Xa-7)和IRBB21(Xa-21)与6个常规感病品种和5个常用不育系及相应保持系配组,对其F1代杂种用病菌致病型Ⅱ和Ⅳ的KS-66和ZJ-173菌株进行接种,发现抗病基因Xa-7和Xa-21在杂种F1中对病菌表现抗病反应,而抗病基因Xa-4在杂种F1中仅表现中抗反应。提出了在水稻抗白叶枯病育种中应加快对显性广谱抗病基因Xa-7和Xa-21的利用
【Abstract】 It was evaluated that the reaction pattern of a set of rice near iso lines with 12 resistant genes to the 10 strains from different districts representing 7 pathotypes of Xanthomonas oryzae pv. oryzae . The results indicated that the genes Xa 4, xa 5, Xa 7, xa 13 and Xa 21 were almost resistant to all 7 Chinese pathotypes in the seedling, tilling and flowering stages. Further the F 1 generation between resistant lines: IRBB4 (Xa 4), IRBB7 (Xa 7), IRBB21 (Xa 21) and susceptible cultivars: TN1, IR24, Guichao2, Minghui63, Shuanggui1 and Zhenzhu′ai as well as susceptible cytoplasmic male sterile lines (or maintainers): D ShanA/B, Zhenshan97A/B, Gang46A/B, Xieqingzao A/B, and You ⅠA/B were inoculated with the strains KS 66 (pathotype Ⅱ) and ZJ 173 (pathotype Ⅳ) to test the expression of three dominant resistant genes in hybrid plants. It revealed that the genes Xa 7 and Xa 21 were more resistant than Xa 4 in F 1 generation of all combination tested. It suggested that application of the resistant genes Xa 7 and Xa 21 should be strengthened during the common and hybrid rice breeding resistant to the bacterial leaf blight.
【Key words】 rice; Xanthomonas oryzae pv. oryzae; disease resistance; gene expression;
- 【文献出处】 南京农业大学学报 ,JOURNAL OF NANJING AGRICULTURAL UNIVERSITY , 编辑部邮箱 ,1997年02期
- 【分类号】S511.103.4
- 【被引频次】28
- 【下载频次】112